The function of restriction endonucleases
(a) Useful in genetic engineering
(b) Protects the bacterial DNA against foreign DNA
(c) Both a and b
(d) Helpful in photosynthesis
Answer
635.4k+ views
Hint: Restrictions endonucleases cut the two strands of DNA at different locations which are used to form recombinant DNA molecules by using various techniques and it also masks the bacterial DNA by adding methyl groups.
Complete answer:
In Escherichia coli, the growth of bacteriophage is restricted by two enzymes. They both were isolated. In those two enzymes, one enzyme adds a methyl group to DNA, and the other enzyme cuts the DNA. The enzymes that cut the DNA are called restriction endonucleases. Their function depends on a specific DNA nucleotide sequence which was isolated and characterized later. To form recombinant molecules of DNA, restriction endonucleases are used in genetic engineering. They also help in protecting the bacterial DNA from foreign DNA by masking their own DNA with methyl groups.
Additional information:
- Nucleases are the larger class of enzymes that contain restriction enzymes. They are of two types namely exonuclease and endonucleases.
- The function of restriction endonucleases depends on the isolated specific DNA nucleotide sequence which was characterized later
- Nucleotides at the end of the DNA are removed by exonucleases and cuts at specific positions within the DNA are done by restriction endonucleases. They function by inception the length of the DNA sequence.
- Molecules of DNA that are composed of DNA from different sources or genomes are formed by using restriction endonucleases in genetic engineering.
So, the correct answer is ‘both a and b’.
Note:
-Each restriction endonuclease can detect a specific palindromic sequence of nucleotides in the DNA.
-Most common restriction endonucleases make the staggered cut which means cutting the two strands of DNA at different locations.
Complete answer:
In Escherichia coli, the growth of bacteriophage is restricted by two enzymes. They both were isolated. In those two enzymes, one enzyme adds a methyl group to DNA, and the other enzyme cuts the DNA. The enzymes that cut the DNA are called restriction endonucleases. Their function depends on a specific DNA nucleotide sequence which was isolated and characterized later. To form recombinant molecules of DNA, restriction endonucleases are used in genetic engineering. They also help in protecting the bacterial DNA from foreign DNA by masking their own DNA with methyl groups.
Additional information:
- Nucleases are the larger class of enzymes that contain restriction enzymes. They are of two types namely exonuclease and endonucleases.
- The function of restriction endonucleases depends on the isolated specific DNA nucleotide sequence which was characterized later
- Nucleotides at the end of the DNA are removed by exonucleases and cuts at specific positions within the DNA are done by restriction endonucleases. They function by inception the length of the DNA sequence.
- Molecules of DNA that are composed of DNA from different sources or genomes are formed by using restriction endonucleases in genetic engineering.
So, the correct answer is ‘both a and b’.
Note:
-Each restriction endonuclease can detect a specific palindromic sequence of nucleotides in the DNA.
-Most common restriction endonucleases make the staggered cut which means cutting the two strands of DNA at different locations.
Recently Updated Pages
Which of the following graphs shows the variation of class 12 physics CBSE

Draw a labelled diagram of the human male reproductive class 12 biology CBSE

Describe the experiment to compare the emf of two cells class 12 physics CBSE

What is standard hydrogen electrode

What is conventional current and electric current class 12 physics CBSE

2Bromopentane is treated with an alcoholic KOH solution class 12 chemistry CBSE

Trending doubts
Draw a labelled sketch of the human eye class 12 physics CBSE

Which are the Top 10 Largest Countries of the World?

A member of Simon commission later became Prime Minister class 12 social science CBSE

Draw ray diagrams each showing i myopic eye and ii class 12 physics CBSE

Give one example of a reptile that is viviparous class 12 biology CBSE

Which is the correct genotypic ratio of mendel dihybrid class 12 biology CBSE

