Automated DNA sequencing is based on a method developed by
A) Erwin Chargaff
B) Maurice Wilkin
C) Francis Crick
D) Frederick Sanger
E) Alec Jeffreys

Hint: Automated DNA sequencer is a scientific instrument that is used to automate the DNA sequencing process, and the DNA sequence is used to determine the order of four bases, such as Guanine, cytosine, adenine, and thymine, and this process is termed as read. Some optical instruments are also used as a DNA sequence that is worked by analyzing light signals originating from fluorochromes.

Complete answer:
1) At first, the DNA sequencing method was developed by Gilbert and Sanger.
2) Gilbert introduced a sequencing method that is based on chemical modification of DNA, followed by cleavage on a specific base pair.
3) Apart from the Gilbert technique, there is another technique called the Sanger technique, which Is based on dideoxynucleotide Chain termination.
4) Among all automated sequencer methods, the Sanger method obtained popularity, due to its increased efficiency and low radioactivity.
5) The first automated DNA sequence was AB370A, where it was introduced in the year 1986 by the applied biosystem, and this one can sequence almost 96 samples per day.
6) After the Sanger automated DNA sequencer, another generation or advanced DNA sequencer was released in 1995, where they utilize a linear polymer in capillary method, place of slab gel for DNA strand separation by electrophoresis.
7) As the person to introduce the DNA sequencer was both Gilbert and Sanger.

Option D is the correct answer.

Note: Automated DNA sequencer is mainly utilized for sequencing the base pairs, and the first one was introduced by Gilbert and Sanger later so many are introduced, but a very recent one is a third generation sequencer, where it does not require any amplification before sequencing.